This perfusion system is the combination of the VC-6MCS Perfusion Valve Control System and the SF-77B Fast Step Perfusion System.
The system includes:
- VC-66MCS - Complete 6 channel mini-valve perfusion system
- SF-77B - Perfusion Fast Step
- MM-33R - Right handed Micromanipulator
- MBB - Magnetic Base
The VC-77SP is a unique system which enables the user to rapidly select between several perfusion reservoirs, saving time and effort. The system is comprised of the VC-66MCS Valve Control System and the SF-77B Perfusion Fast Step.
The main use of the SF-77B is the rapid change of solutions bathing a single cell or membrane patch attached to a patch electrode. This is typically achieved using a three square barrel piping system, although up to five barrels can be easily accommodated as well as two barrel Theta glass tubing.
Solution changes can be made in as little as 20-50 ms (or as little as ≈ 1 ms using a two-barrel Theta tubing), and once changed the new solution with its specific concentration of pharmacological agent will remain stable until changed again.
An important capability of this device is that a large number of solutions can be used during the course of a single experiment, regardless of the compact size of the device. This creates the ability to use 15-30 different solutions in a single experiment. This feat is accomplished by having as many as six solutions feeding through a unique manifold into each of the three "pipes" in front of which the cell may be placed. This allows any of the three pipes to have any one of six solutions flowing through it at any time. It is even possible, during the course of an experiment, to change the six solutions flowing into any one of the manifolds, allowing a virtually limitless number of solutions to be used.
The VC-6M Valve Control System lies at the heart of a multi-valve perfusion system designed to automate and control the delivery of small quantities of fluids to Warner Instruments imaging and recording chambers. However, its flexible design allows the system to be used in many applications not using Warner equipment. The miniature solenoid valve assembly is standard with the system. They are lightweight and made from rugged materials for long life.
Valves are dual acting with both normally open and normally closed sides. The normally open side is plugged with removable stoppers and when these are removed solution can flow to waste while the valve is in the off position.
The controller can independently regulate the function of up to six valves. Individual valves can be controlled via a manual switch or an external digital (TTL) signal. Valve transitions (opened or closed) occur at full power to insure rapid response times and are then held in place at less than half power to prevent heat transfer to solutions. An event-marker pulse, generated each time a valve is switched on, is provided at the rear of the controller for recording into your acquisition system.
Valves 12 V DC /0.046 A, solenoid type
Valve manifold Teflon material, mounts on a 3/8" or 1/2" ring stand
Connection Cable 2.4 meter (8 ft.) connecting cable terminated with quick disconnects on valve end and 15 pin male "D" type connector on controller end.
Reservoirs 10 cc capacity syringes
Reservoir Holder Delrin. Holds eight syringes with thumb screws for each reservoir.
Valve Controller Typical response times for valves is 2.5 ms
Switch Selection Manual, Off or External
External Input +5 V TTL-compatible (BNC Connector)
Event Marker Logic level pulse 500 ms nominal (rear panel BNC connector)
Manifold 2-to-1, 4-to-1, or 6-to-1. Nominal dead space
Power 110 - 130 or 200 - 250 V AC, single-phase, 50/60 Hz, 20 W
Operating Temperature 10 - 40 °C (50 - 104 °F)
Dimensions (H × W × D) 89 × 203 × 305 mm (3.5 × 8.0 × 12 in.)