MOM

Vielseitiges Zweiphotonen- und Weitfeld-Mikroskop (Sutter Instrument)
Beschreibung

Twophoton and Wide Field Microscope

Features

  • Objective moves 22 mm in X, Y and Z
  • Objective rotates about optical axis for imaging of non-horizontal surfaces and volumes
  • Customizable open platform design
  • Cambridge Technology XY scanners
  • Two or four channel detector system with Hamamatsu PMTs and preamplifiers
  • Sutter PS-2 / PS-2 LV dual channel PMT power supply
  • National Instruments based data acquisition system

The Movable Objective Microscope (MOM) is a two-photon microscope capable of imaging deep within living specimens when combined with a Ti:Sapphire laser. The MOM design is unique in providing 3-dimensional objective movement and rotation allowing the specimen to remain stationary. Many highly regarded imaging laboratories around the world use MOM microscopes and we constantly work with our customers to adapt the design for their changing needs.

Starting in 2011, Sutter has begun offering the MOM Computer System with software (MCS). The MCS is designed to take advantage of the MOM. As a result, when combined with MCS, the MOM becomes a powerful tool capable of combining electrophysiology with large scale imaging experiments in both two and three dimensions.

How it works:
The MOM consists of two independent microscopes. The widefield half of the microscope consists of an Olympus vertical illuminator, Sutter Xenon arc lamp and camera mount to provide standard epifluorescence. The two-photon side of the microscope provides the optical pathway for guiding the excitation laser light from the table up into the scanning galvanometric mirrors and then expanding the beam through the scan lens and directed into the back of the objective. Following two-photon excitation, the emitted photons are directed by a dichroic mirror immediately above the objective into the detection pathway. The main body of the microscope translates on a rail system to allow easy access to the specimen prior to imaging.

The objective translates in X, Y and Z as well as rotates around the X axis. Two moving mirrors allow the microscope to maintain efficient delivery of the excitation light to the back aperture of the objective regardless of movement or orientation. The X,Y and Z movements used are the same as that in Sutters MP-285 micromanipulator so you know the movements are smooth, fine in scale, drift-free and highly reproducible. These movements permit Z-stacks and mosaic images of large regions of tissue to be recorded without the need for a moving stage.

The horizontal light path allows for rotation of the objective away from the standard vertical position. As a result of this rotation, the MOM can easily be converted from an upright to an inverted microscope and the objective positioned from 0 to 180 degrees. This positional freedom permits the imaging of nonhorizontal surfaces and volumes.

  • Sutter MOM packages include all of the equipment (less the Ti:Sapphire laser and objective), needed for a complete imaging system.
  • Cambridge Technology XY galvanometric scanners with 3 or 6 mm mirrors
  • Hamamatsu photomultiplier tubes (PMTs): R6357 multialkali or H10770PA-40 (GaAsP) products.   Sutter is an authorized dealer for Hamamatsu
  • Power supplies for PMTs: Either a Sutter PS-2 (dual channel high-voltage power supply for  R6357 PMTs) or Sutter PS-2LV (dual channel low-voltage power supply for H1070 (GaAsP) PMTs) can be ordered.
  • Hamamatsu C7317 or Sigmann pre-amplifiers
  • Data acquisition: National Instruments acquisition boards (NI-611E)

 

Specifications

MOM AND CONTROLLER
Travel     22 mm on all three axes
Resolution     MP-285 controller         Low: 0.2 µm/step    
Hight: 0.04 µm/step
MPC-200 controller      0.0625 µm/step
Maximum Speed     MP-285 controller      2.9 mm/s
MPC-200 controller       5.0 mm/s
Long Term Stability     1-2 µm/h
Drive Mechanism     Precision worm gear capstan drive
Communication     MP-285      RS-232 Serial
MPC-200     USB
Electrical     115/230 Volts  50/60 Hertz power line

LAMBDA LS  300W XENON ARC LAMP
Lamp Life     1,000 h  (500 h warranty)   Longer life depends on application
Electrical      115/230 Volts  50/60 Hertz power line

PS-2/PS-2LV  PMT POWER SUPPLY
Electrical      115/230 Volts  50/60 Hertz power line
   
MDR-6    SCAN DRIVE CONTROLLER
Electrical      115/230 Volts  50/60 Hertz power line


The MOM is both an inverted and upright microscope with 0 to 180° rotation

 

Applications

  • In vivo two-photon imaging
  • Patch-slice, fixed-stage microscopy
  • Non-horizontal surface microscopy
  • Simultaneous retinal stimulation and two photon microscopy
    (see  Euler et al., "Eyecup scope-optical recordings of light stimulus-evoked fluorescence signals in the retina", Pflügers Arch., 2008.

 


2 channel


4 channel


Short path (2 channel)

 

Ordering information

MOM BASIC SYSTEM FOR 2-PHOTON MICROSCOPY
Includes Moving Objective Microscope, 2 channel detector with PMTs, preamps and PS-2 Power supply, XY scanners with servo drive, wide field fluorescence unit
including vertical illuminator, Lambda LS 300 Watt Xenon Arc lamp, LLG and light guide adapter, C-mount for wide field camera, data acquisition system

MOM-3MM    MOM System with 3 mm XY scanners
MOM-6MM     MOM System with 6 mm XY scanners

Final pricing depends on detector path selected and does not include several devices necessary for a complete 2-photon microscope (i.e. Ti:Sapphire laser, objective, camera, trinocular head, table mount optics).

 

Accessories

MOM software

MCS

MOM Computer System
and Software
 

Mouse Head Holding Device MAG-2

MAG-2

Head Holding Device
with Angle Adjuster for Mice
(Narishige)