RC-3Z

A simple low-cost chamber for studies of oocytes and other large cell structures.

• Specially designed for oocyte studies
• Features two slot-shaped bath wells
• Allows fast solution exchange and easy cell access
• Adjustable solution height

The RC-3Z features two slotted bath wells; a narrow bath for applications requiring rapid solution exchange, and a wider bath allowing good electrode access.

The chamber is constructed using a two-piece design.  The top piece forms the chamber body and contains a solution aspiration reservoir.  The bottom piece forms the floor of the chamber and houses the oocyte work area.  An agar bridge well with connecting channel to the input side of the bath is also provided. An isolated well is provided on the downstream side of the bath, however, most users place the sense electrode directly into the suction well.

The perfusion inputs accept standard 1/16" OD (PE-160) polyethylene tubing.  Solutions are removed from the suction well via an adjustable suction tube and solution height is set by raising or lowering this suction tube. The suction tube out-port connects to 1/16" ID tubing.  Threaded holes are incorporated in the chamber body for mounting of the OC-725C bath headstage.

Chamber material
    polycarbonate

Footprint (L × W)
    13.0 × 7.7 cm

Chamber floor insert
    25 × 75 × 1 mm, polycarbonate

Narrow bath dimension (L × W × H)
    27 × 3.2 × 3.2 mm

Narrow bath volume, by depth
    85 μl/mm

Wide bath dimension (L × W × H)
    27 × 6.3 × 3.2 mm

Wide bath volume, by depth
    170 μl/mm

Input tubing (ID × OD)
    1.14 × 1.57 mm (PE-160)

Aspirator coupling
    1.67 mm (OD)