XenoWorks™ System

XenoWorks™ is a modular microinjection system designed to meet a wide variety of application needs. SUTTER´s experience with precision motor control has culminated in this state-of-the-art system. The XenoWorks™ Micromanipulator uses a classic inverted joystick design for excellent ergonomics, intuitively linking the user with the micropipette. Combined with smooth, responsive stepper-motor drives of Sutter Instrument, the XenoWorks™ Manipulator provides exceptional mechanical stability, sensitivity, and range of movement.

The XenoWorks™ Digital Microinjector was designed for precision manipulation of cells via an easy-to-use interface. The Digital Microinjector employs a self-contained vacuum channel for gentle suspension-cell holding applications. A second channel is available for both high-pressure injection and gentle positive/negative pressure for transfer of embryonic stem cells. Both injection duration and pressure are easily selectable using rotary knobs on the remote interface. The internal low-speed compressor removes the need for an external pressure supply. The XenoWorks™ family also includes the Analog Microinjector for simple cell holding and transfer applications, which incorporates the same smooth response and ergonomic design of our other XenoWorks™ products.

TYPICAL APPLICATIONS

• Pronuclear microinjection of DNA into mouse zygotes
• Embryonic stem cell transfer into blastocysts
• Somatic cell nuclear transfer
• Intracytoplasmic sperm injection (ICSI)
• Piezo-assisted sperm-mediated transgenesis and nuclear transfer
• Please contact us for suitability of XenoWorks for applications not listed here

SYSTEM FEATURES

XENOWORKS MICROMANIPULATOR

Height- and tension-adjustable inverted joystick for optimal ergonomics

Exceptionally smooth and responsive micropipette movement

Variable movement range - 6 settings from coarse to ultra-fine

User definable memory positions, including “Work” and z-axis lower limit

A wide 25 mm of travel in all three axes

XENOWORKS DIGITAL MICROINJECTOR

Independent “hold” and inject/transfer channels

Up to 5 600 hPa of injection pressure

Remote keypad with rotary controls and high visibility

LED displays

XENOWORKS ANALOG MICROINJECTOR

Highly stable, drift free

Coaxial coarse and fine displacement (10 : 1 ratio) for fast filling and precision control

Use with a variety of hydraulic fluids, including oil, air and water

Interchangeable syringes (500 µl standard, 100 µl and 1000 µl options)

Specifications

XenoWorks MICROMANIPULATOR INPUT DEVICE

Height- and resistance-adjustable inverted joystick with integral declutch, 6 position rotary range control, low-profile base

RANGE SETTINGS (ONE FULL JOYSTICK SWING) 12 mm; 3.3 mm; 880 µm; 400 µm; 200 µm; 100 µm

MICROPIPETTE POSITION MEMORY 1 user-defined set-point – “Work”; 1 user-defined z-axis limit – “Floor”; 2 preset points – “Home” and “Setup”

MAXIMUM MOTOR SPEED / TRAVEL RANGE 5.5 mm/s / 25 mm

MICROPIPETTE HOLDER GRIP 4 mm outer diameter

ELECTRICAL 115/230 V 50/60 Hz

XenoWorks DIGITAL MICROINJECTOR

FUNCTIONS Hold, Inject, Transfer, Clear

PRESSURE CHANNELS 1 Hold, 1 Inject/Transfer

PRESSURE RANGE Hold: -350 to +350 hPa, 7 hPa steps
Inject: 0 to 5600 hPa, 7 hPa steps
Transfer: -175 to +175 hPa, 7 hPa steps

INJECTION DURATION Variable: 0.01 to 10 s in 0.1 s increments

INJECTION ACTIVATION Hand- or foot-switch

MICROPIPETTE COMPATIBILITY 1 mm O.D. capillary glass (other sizes available upon request)

ELECTRICAL 110 or 220 V (please specify) 50/60 Hz

XenoWorks ANALOG MICROINJECTOR

COARSE : FINE RATIO 10:1

INTERCHANGEABLE SYRINGE RESERVOIR 1000µl, 500µl, 100µl

DISPLACEMENT / TURN 500µl syringe – 5.2 µl coarse, 0.5 µl fine
1000 µl syringe – 10.4 µl coarse, 1.04 µl fine

TUBING 1 m PTFE

MICROPIPETTE HOLDER Diameter: 4 mm
Accepts: 1 mm O.D. capillary glass (other sizes upon request)

ELECTRICAL 115/230 V 50/60 Hz