BOB
The Sutter BOB™ — designed to eliminate the conventional microscope frame — is a simple, open-design upright microscope platform ideal for slice electrophysiology, wide field functional imaging, two photon retinal imaging, photostimulation and new techniques just being developed! A microscope, in its simplest form, is an objective and a tube lens. Other components of most modern microscopes are designed to serve specific functions: different types of experiments, methods of illumination and means of signal detection.
Replacing the microscope frame with an optical rail builds in the ability to adjust the overall height of the microscope, unheard of in conventional microscope designs. Work on slices in January, do in vivo experiments in March. The BOB microscope is a compact, single assembly that mounts to the “blue rail” with one massive, stable connection. Focusing is motorized and incorporated between the focus arm and the optical rail.
Fluorescence epi-illumination is built into the basic BOB via an Olympus vertical illuminator. LED transmitted light illumination uses the Olympus Oblique Coherent Contrast (OCC) condenser. Sutter’s TLED and TLED controller form the trans-illumination light source. The TLED controller is capable of being triggered with a digital signal eliminating the need for shutters and adding the ability to photostimulate from the trans location. In experiments where transmitted light is not desired, the LED, condenser focus mechanism and OCC condenser are easily removed as a single assembly. Additionally, the transmitted light path is shorter than in other frames, allowing the microscope body to sit significantly lower than a conventional microscope. When the microscope is shorter, there is more stability, increased ergometrics and ease of use.
The Sutter BOB when configured with an optional motorized XY stage or translator with MPC-200 controller, takes full advantage of our free Multi-Link™ software program for micromanipulator positioning. During whole-cell patch recording in slices, it is common to search a large area of the slice to find appropriate neurons. If the BOB is configured with Multi-Link, after you find your target, Multi-Link will then retrieve your recording and stimulation pipettes to the same field of view so that you can begin recording immediately. If later you need to stimulate a region outside the current field of view, Multi-Link can release the recording pipette and allow you to reposition the objective and stimulating pipette(s) to the new stimulation region.
Applications
- in vivo and in vitro
- Whole-cell imaging
- Intracellular imaging
- Material science
FEATURES
- Optional motorized fixed XY stage or motorized translator
- Open design microscope with motorized focus
- Quickly configurable based on experimental needs
- Optimized to allow in vivo and in vitro experimentation on one setup
- Designed for use with Olympus objective lenses
- Free Multi-Link™ software coordinates movement with micropipette positioning of MPC-200
- Oblique Coherent Contrast (OCC) or Differential Interference Contrast (DIC)
- Epi-fluorescent illumination
OPTIONS
BOB systems with fluorescence
BOB-FL BOB microscope with epi-illuminator
BOB-FLTL BOB with epi-illuminator, transmitted light
BOB-FLTL2 BOB with epi-illuminator, dual transmitted light
BOB-FLTR BOB with epi-illuminator, trinocular head
BOB-FLTRTL BOB with epi-illuminator, trinocular head, transmitted light
BOB-FLTRTL2 BOB with epi-illuminator, trinocular head, dual transmitted light
BOB systems without fluorescence
BOB basic BOB microscope system
BOB-TL BOB with transmitted light
BOB-TL2 BOB with dual transmitted light
BOB-TR BOB with trinocular head
BOB-TRTL BOB with trinocular head, transmitted light
BOB-TRTL2 BOB with trinocular head, dual transmitted light
OBJECTIVES
The BOB uses a standard Olympus 2-position nosepiece. You can use your own objectives or select up to 2 from the choices below.
OBJ-522 4X-UPLFLN4X; U PLAN FLUORITE 4× OBJECTIVE; NA 0.13, WD 17 mm (recommended for electrophysiology)
OBJ-342 5X-LMPLFLN5X; LWD M PLAN FL 5× OBJECTIVE; NA 0.13, WD 22.5 mm
OBJ-343 10X-LMPLFLN10X; LWD M PLAN FL 10× OBJECTIVE; NA 0.25, WD 21 mm
OBJ-524 10X-UPLFLN10X2; U PLAN FLUORITE 10× OBJECTIVE; NA 0.3, WD 10 mm (recommended for electrophysiology)
OBJ-824 10X-UPLSAPO10X2; U PLANS S-APO 10× OBJECTIVE; NA 0.4, WD 3.1 mm
OBJ-525 20X-UPLFLN20X; U PLAN FLUORITE 20× OBJECTIVE; NA 0.5, WD 2.1 mm
OBJ-585 20X-UMPLFLN20XW; LWD PLAN FLUOR 20× WTR DIPPING OBJ; NA 0.5, WD 3.5 mm
OBJ-766 20X-UAPON20XW340; UAPON 20× WATER 340 nm OBJ; NA 0.7, WD 0.35 mm
OBJ-825 20X-UPLSAPO20X; U PLAN S-APO 20× OBJECTIVE; NA 0.75, WD 0.6 mm
OBJ-527 40X-UPLFLN40X; U PLAN FLUORITE 40× OBJECTIVE; NA 0.75, WD 0.51 mm
OBJ-587 40X-LUMPLFLN40X/W; U M PLAN FLN 40×/W OBJ; NA 0.8, WD 3.3 mm, IR, ECO (recommended for electrophysiology)
OBJ-769 40X-UAPON40XW340; UAPON 40× WATER OBJ; NA 1.15, WD 0.25 mm, W/CC, BFP1
OBJ-350 50X-LMPLFLN50X; LWD M PLAN FL 50× OBJECTIVE; NA 0.5, WD 10.6 mm
OBJ-592 60X-LUMPLFLN60X/W; U M PLAN FLN 60×/W OBJ; NA 1.0, WD 2.0 mm, IR, ECO (recommended for electrophysiology)
OBJ-672 60X-LUMFLN60X/W; NA 1.1, WD 1.5 mm, 25 ANGLE, IR.CC, ECO (recommended for electrophysiology)
OBJ-832 60X-UPLSAPO60XO; U PLAN S-APO 60× OIL OBJ; NA 1.35, WD 0.15 mm, BFP
OBJ-893 60X-UPLSAPO60XW; U PLAN S-APO 60× W OBJ; NA 1.2, WD 0.28 mm, W/CC 0.13-0.21
CONDENSORS
For models with TL in the part number, choose a condenser. For DIC models, select the condenser that matches the highest power of your selected objectives.
OCC Oblique coherent condenser
DIC-40X DIC condenser for BOB with 40× objective
DIC-40XIR DIC condenser for BOB with 40× objective and IR
DIC-60X DIC condenser for BOB with 60× objective
DIC-60XIR DIC condenser for BOB with 60× objective and IR
FILTER CUBES
For models with FL in the part number, choose a filter cube(s) and a light source.
CUBE-GFP Filter Cube - GFP
CUBE-CY3 Filter Cube - CY3
CUBE-YFP Filter Cube - YFP
CUBE-DAPI Filter Cube - DAPI
CUBE-A635 Filter Cube - ALEXA 635
Many more filter cubes available on request.
LIGHT SOURCES
HPX-L5 Lambda HPX-L5 High powered LED light source
LS-OF30 Lambda LS with 300 Watt ozone free lamp
LB-421 Lambda 421 Optical Beam Combiner and controller
LB-OBC Lambda OBC Optical Beam Combiner
FLED-BOB Lambda FLED for BOB
FLED-DC-BOB Dual Channel Lambda FLED for BOB
LG-BOB Light guide adapter
ACCESSORIES
Moving Platforms
(Please specify chamber type when ordering)
MPC-88 Moving platform stage plate with motorized gantry supports, MPC-200 controller and ROE, cables and manual
MPC-88/M6 Same as MPC-88 except with M6 tapped holes
MPB-88 Moving platform stage plate with motorized gantry supports, BOB controller, cables and manual
MPB-88-M6 Same as MPB-88 except with M6 tapped holes
MP-88 Moving platform stage plate with motorized gantry supports, MP-285 controller and ROE, cables and manual
MP-88/M6 Same as MP-88 except with M6 tapped holes
common MP-88 / MPC-88 Chamber inserts
(no charge when ordered with MP-88 or MPC-88)
X040500 Warner Series 20 (round) - aluminum
X040503 Warner Series 20 (round) - stainless steel
X040505 Warner Series 20 (octagonal) - aluminum
X040507 Warner Series 20 (octagonal) - stainless steel
X040510 50 mm round - aluminum
X040512 50 mm round - stainless steel
X040497 Blank insert - Aluminum
X040498 Blank insert - stainless Steel
SI-SLIDE Insert with slide holder
SI-W30 Warner Series 30 platform - aluminum
SI-W30ST Warner Series 30 platform - stainless steel
SI-ALA-MS ALA MS series insert - aluminum
other MP-88 / MPC-88 Chamber inserts
SI-PETRI for Petri dish and slides
SI-CUSTOM custom stage insert
Motorized Translator and Stands
MT-2000-BOB Motorized translator with MP-285 controller, ROE, and stands
MT-2200-BOB Motorized translator with MPC-200 controller, ROE, and stands
MT-2800-BOB Motorized translator with BOB controller, ROE, and stands
Motorized Translator and Fixed Platform Stage
MT-2088-BOB Motorized translator with MP-285 controller, ROE, and fixed platform stage
MT-2288-BOB Motorized translator with MPC-200 controller, ROE, and fixed platform stage
MT-2888-BOB Motorized translator with BOB controller, ROE, and fixed platform stage
Motorized Translator only (no stands or platform)
MT-800-BOB Motorized translator with MP-285 controller and ROE
MT-820-BOB Motorized translator with MPC-200 controller and ROE
MT-880-BOB Motorized translator with BOB controller and ROE
Camera
CAM-EL ELECTRO, Cooled 1.4 MP CCD Camera
CAM-PR PRIME 25B, Back illuminated CMOS Camera
CAM-BSI PRIME BSI, High-res scientific CMOS Camera