The P-2000 micropipette puller represents a significant advance in the technology of fabrication of micropipettes, optical fiber probes, and nanospray tips. The P-2000 integrates a CO2 laser-based heat source with the technology derived from our extensive experience with conventional pullers. This system offers capabilities unmatched by other pullers.
While the P-2000 is suitable for working with most conventional glasses, its primary advantage is the ability to work with quartz (fused silica) glass. Quartz offers superior material properties for a variety of research applications. Quartz is stronger than other glasses and can facilitate penetration through tough tissues which would normally break conventional pipettes1. For applications requiring a low noise glass, users will find that quartz is the lowest noise glass available2,4. Quartz contains none of the metals used in conventional glasses3. Optically, quartz is virtually free from fluorescence when illuminated.
A CO2 laser was selected as the heat source for the P-2000 for several reasons.
The P-2000 can store up to 100 separate programs, with each program consisting of up to 8 command lines. Programmable parameters include heat, pull strength, velocity (criterion for the trip point) filament type, and delay (time between the trip point and the hard pull).
One important consideration for the use of the P-2000 is the diameter of the glass used. The optical design produces even heating on glass up to 1.2 mm in outside diameter. Larger diameter glasses can be used with the P-2000 (up to 1.5 mm quartz and 1.8 mm conventional glasses), but the performance is best with glass that is 1.2 mm diameter or less.
The P-2000 works well with small diameter glasses such as HPLC tubing and optical fibers. These smaller diameter glass configurations (0.125 mm > outside diameter < 0.6 mm ) require the installation of some modified components including special glass clamping bars.
These modified components will be installed, if requested, at the time of purchase. As with larger diameter glass, a wide range of tip sizes and taper geometries can be produced with this modified P-2000 and small diameter glass. We have drawn optical fiber tips ranging from less than 10 nm to more than 0.5 nm. Please consult our technical staff for further information.
Dimensions 94 cm × 48 cm × 48 cm
Weight 43 kg
Electrical 115/230 V, 50/60 Hz
for all Sutter Instrument