RC-1Z

A simple, low cost oocyte perfusion chamber

• Specially designed for oocyte studies
• Unique slotted bath
• Dimpled bottom to secure oocyte into position

The RC-1Z chamber features a unique slot-shaped bath for applications requiring rapid perfusion.

The chamber is constructed using a two-piece design.  The top piece forms the chamber body and contains a solution aspiration reservoir.  An agar bridge well with connecting channel to the input side of the bath is also provided.  An isolated well is provided on the downstream side of the bath, however, most users place the sense electrode directly into the suction well.

The bottom piece forms the floor of the chamber and houses the oocyte work area.  To aid in securing the oocyte into position during impalement, the chamber bottom has three dimples with diameters of 0.8, 0.9, and 1.0 mm.  Three grooves milled into the chamber bottom (parallel to the direction of solution flow) aid in bathing the bottom side of the oocyte during perfusion.

Solutions are removed from the suction well via an adjustable suction tube and bath solution height is adjusted by raising or lowering the suction tube.  The perfusion input accepts standard 1/16" OD (PE-160) polyethylene tubing.

Material
    polycarbonate

Footprint (L × W)
    7.7 × 5.1 cm

Bath Dimensions (L × W × H)
    25 × 3.5 × 2.5 mm

Volume, by depth
    85 μl/mm

Chamber Floor Insert
    25 × 75 × 3 mm, polycarbonate

Input Tubing (ID × OD)
    1.14 × 1.57 mm (PE-160)

Aspirator coupling
    1.67 mm (OD)