Beschreibung

ACC-BC25-MIT-200-1 Microscope illumination tube for coupling beam combiner/LCS into the infinity optical path of microscopes. Length designed for one(1) beam-combiner distance (up to two LCS sources).

ACC-BC25-MIT-200-2 Microscope illumination tube for coupling beam combiner/LCS into the infinity optical path of microscopes. Length designed for two(2) beam-combiner distances (up to four LCS sources).

IMPORTANT : (1) LED’s can ONLY be driven by a constant-current source, and NOT a voltage source (e.g. a battery, or a AC/DC power supply etc.); (2) Please always verify LED’s current rating first before applying current to the LED, and please always make sure NOT to apply current that is above the LED’s current rating.

About Mightex High Power LED Light Sources

Selecting a Microscopy LED Light Source

1. Select LED Wavelength

Emission Spectra — Multiple Available Wavelengths (Click to enlarge. Also see: Specification Sheet; Manufacturer's website)

2. Select LED Configuration

Mightex Microscope LED Light Source Configuration — Single-Wavelength: **Type A, Type B, Type J & Type H**

3. Select LED Controller

Mightex Microscope LED Light Source Controller Option — Manual/Analog: BLS-1000-2, BLS-3000-2, BLS-13000-1, and BLS-18000-1

4. Microscope Coupling

Microscope adapters are available for all major manufacturers (Nikon, Olympus, Leica, Zeiss). If you have a different microscope, please contact us.

LED Application Examples

Abdelfattah AS et al., Bright and Photostable Chemigenetic Indicators for Extended In Vivo Voltage Imaging – Science (2019)
Abdelfattah et al., developed novel chemigenetic indicators for in vivo voltage imaging in neurons. To illuminate their indicators, the group used Mightex’s Microscope LEDs.

Crandall SR, et al., Infrabarrels Are Layer 6 Circuit Modules in the Barrel Cortex that Link Long-Range Inputs and Outputs – Cell Reports (2017)
In this work, Crandall et al., used Mightex’s Microscope LEDs to optically stimulating different neuronal pathways connecting to pyramidal neurons found in L6 of the whisker somatosensory cortex of mice.

Higgs MH, Measurement of Phase Resetting Curves Using Optogenetic Barrage Stimuli – Journal of Neuroscience Methods (2017)
To study phase resetting curves in vivo, Higgs and Wilson used Mightex’s Microscope LEDs to establish whether their novel method of optogenetic barrage stimulation would work during extracellular spike recordings (a recording method often used in in vivo experiments).