The MED64 Presto is a microelectrode array (MEA) system that incorporates the industry leading sensitivity of the MED64 platform in an easy to use multi-well format. Modern, intuitive, easy to use software makes data collection and analysis effortless.

The MED64 Presto is engineered for researchers who need the correct answer to their scientific inquiries and reveals data that can be missed by other platforms.

Features

• Flexible 6, 24, 48, and 96 multi-well formats.
• Industry leading high-sensitivity, low-noise system (0.9 µV;  < 3 kHz), providing excellent signal-to-noise.
• New carbon nanotube electrodes provide a friendly substrate for cells to adhere.
• Transparent glass base allows clear visibility of cells and compatibility with imaging.
• Innovative well design (sakura), and ready-to-use accessories that help plating cells.
• Integrated stage heater and environmental chamber.
• Modern, state of the art software.

High-sensitivity Electrodes in Multi-well Format

The MED64 Presto’s electrodes are engineered for sensitivity to reveal data that can be missed by other systems. Better sensitivity mean more signals can be detected from your cells. Presto’s new carbon nanotube technology provides a flatter electrode surface compared to traditional MEA electrodes resulting in better cell adhesion onto the electrodes.

Applications

• In vitro Micro-electrode array assay for evaluating in vivo-like convulsive activity in hiPSC-derived neuron and astrocyte co-culture
• Functional characterization of human induced pluripotent stem cell-derived sensory neurons using a high-sensitivity micro-electrode array
• Neurotoxicology screening using a high-sensitivity MEA platform

iPSC neurons cultured on the microelectrodes in the MEA plate (top) and recorded raw data (bottom)

Varieties of MEA Plates

The MED64 Presto’s MEA plates are available with 6, 24, 48, and 96 well format. Transparent plates allow for easy viewing of cells. The 24-well plate has 3 types of well shapes. Sakura plate is engineered for easy and accurate seeding of cells. CellSpotter cartridge is available to help your cell seeding both for eco and comfort plate.

MEA 24well Plate-eco (left) and MEA 24-well Plate-comfort (right)

CellSpotter-comfort (left) and the CellSpotter installed on the MEA Plate (right)

MEA 24-well Plate-sakura (left) and zoom-up vies of a well (right)

MEA Plate options

Software

MEA Symphony: The navigator for your experiment and analysis!

MEA symphony

• Navigate your experiments from setting up experimental assays to producing an analysis report.
• Automated spike detection and subsequent spike and burst analysis.
• Easy and quick set up for analysis parameters as well as manual setting is available.
• Observe all analysis during and post-recording, giving you correct answers to your scientific inquiries.

Specifications

Amplifier
Number of Channels     384
Gain     1000× (fixed)
Sampling Rate     20 kHz
RMS Noise     1.2 μV (< 5 kHz);  0.9 μV (< 3 kHz)
High Pass Filter (LCF)     0.1Hz (fixed)
Low Pass Filter (HCF)     5 kHz (fixed)
Output Channel     2 (for Trigger output)
Stimulation, Output     Voltage driven
Stimulation, Output electrode     2 / 1 well
Stimulation, Max. output voltage     ±2 V_p

Electrode, Well    
Electrode Material     Carbon nanotube
Electrode Size     50 μm × 50 μm
Electrode Impedance     7 kΩ (typ.)
Insulation Layer     Polyimide
Material, Substrate:     Glass
Material, Well:     Acrylic resin
Material, Lead:     ITO

Temperature Controller    
Heater     Trangistor × 4
Sensor     Sensor IC
Resolution     ± 0.1 °C
Temperature Controller     Omron EG5C

Also See:

Product Brochure
( PDF)